Post by / seos988jarvis / 12.09.2019 How to Match the Pipette Tip with Your Experiment? Experiments are all about accuracy and precision. Without these two qualities, any and all results derived from experiments are null and void. The achievement of these qualities in an experiment is partly attributable to the mindfulness of the person carrying out the experiment, the quality of their samples and, or, data as well as the equipment used. More specifically, let us focus on the pipette. This is only useful when it has the right calibrations for the experiment as well as the right tip. Yes, the tip does matter. With that said, we shall look at how to discern whether the yellow pipette tips are better for the experiment or do you need a completely different one? Is it The Right Fit? The fit of the yellow pipette tips does play a major role in determining the accuracy and precision of your pipette. See an ill-fitting tip means that the quantities of reagents used in your experiment will most likely be the wrong ones. Consider the Quality The quality of the tip will directly affect the quality of the results you receive. If there is a variation in quality then you can likely expect a variation in accuracy and precision. Good quality tips tend to be rather standardized and thus safer to use. Specific or Universal? We are often tempted to go with specific tips that, in some instances, come with the pipette from the manufacturer. However, your safest bet would be to make use of universal tips. The latter is designed in such a way that they are compatible with a number of pipettes regardless of the manufacturer. They are also designed to sit perfectly such as not to let out any air and, therefore, do not compromise on the accuracy and precision of the pipette. Filter or No Filter Tips that have no filter (barrier) are generally meant for use in lab experiments that do not require any corrosive, volatile or viscous reagents. If your experiment will require any reagents that fit within the above descriptions then a tip with a filter is best suited. Another plus side to tips without filters is their fair price point. They can also be purchased in bulk and come pre-racked or in reloads that are easy and convenient to use. The only downside is their lack of sterility which can always be corrected by the use of an autoclave. Filter tips on the other hand help to not only safeguard the quality of the experiment but that of the pipette as well. While they are costlier than their non-filter counterparts, they have the advantage of being sterilized upon arrival. If you are new to using pipettes, these are probably the best tips to use as you get the hang of things. Consider the Retention Power You ideally want a tip that is not good at keeping the liquid inside. That said, always look for tips with a low retention ability. This quality of the tips is attributable to the use of plastic that is hydrophobic meaning that it naturally keeps liquids from adhering to its surface. Remember, whether going for yellow pipette tips or universal ones ensure to keep in mind the type of experiment and its needs. Share:
